1) The adhering to are measures used to make DNA fingerprints. What is the third step?

A) collection DNA.

You are watching: Self-replicating dna used to transmit a gene from one organism to another is a

B) Digest v a restriction enzyme.

C) carry out electrophoresis.

D) Lyse cells.

E) add stain.


2) How countless pieces will EcoRI create from the plasmid presented in figure 9.1?

A) 1

B) 2

C) 3

D) 4

E) 5


3) In number 9.1, after ~ digestion v the appropriate restriction enzyme, what is the smallest piece containing the entire ampicillin-resistance (amp) gene?

A) 0.17 kbp

B) 0.25 kbp

C) 1.08 kbp

D) 1.50 kbp

E) 3.00 kbp


4) In figure 9.2, the enzyme in action 1 is

A) DNA polymerase.

B) DNA ligase.

C) RNA polymerase.

D) turning back transcriptase.

E) spliceosome.


5) In number 9.2, the enzyme in step 2 is

A) DNA polymerase.

B) DNA ligase.

C) RNA polymerase.

D) turning back transcriptase.

E) spliceosome.

6) The reaction catalytic analysis by reverse transcriptase is

A) DNA → mRNA.

B) mRNA → cDNA.

C) mRNA → protein.


E) tRNA → mRNA.

7) which of the complying with is an advantage of utilizing E. Coli to do a human gene product?

A) Endotoxin may be in the product.

B) the does not secrete most proteins.

C) that is genes room well known.

D) it cannot process introns.

E) Endotoxin may be in the product and it does no secrete most proteins.

8) which of the following is not an agricultural product make by DNA techniques?

A) frost retardant

B) Bacillus thuringiensis insecticide

C) nitrogenase (nitrogen fixation)

D) glyphosate-resistant crops

E) pectinase

9) If you have inserted a gene in the Ti plasmid, the following step in genetic design is

A) change of coli with Ti plasmid.

B) splicing T DNA into a plasmid.

C) revolution of an pet cell.

D) inserting the Ti plasmid into Agrobacterium.

E) inserting the Ti plasmid right into a tree cell.

10) Biotechnology involves the

A) use of microorganisms to make preferred products.

B) usage of pet cells to do vaccines.

C) breakthrough of disease-resistant chop plants.

D) usage of microorganisms to make preferred products and the use of animal cells to do vaccines.

E) usage of microorganisms to make preferred products, the use of pet cells to make vaccines, and also the advance of disease-resistant crop plants.


The figure at the left in figure 9.3 mirrors a gene determined by southerly blotting. What will a southerly blot that the exact same gene look choose after PCR?

A) a

B) b

C) c

D) d

E) e

12) i m sorry of the adhering to is not a wanted characteristic that DNA vectors provided in gene cloning procedures?

A) self-replication

B) huge size

C) has actually a selectable marker

D) circular kind of DNA or integrates into the organize chromosome

E) might replicate in several species


In number 9.4, the bacteria reinvented with the recombinant plasmid and plated top top media include ampicillin and also X-gal will

A) form blue, ampicillin-resistant colonies.

B) form blue, ampicillin-sensitive colonies.

C) kind white, ampicillin-resistant colonies.

D) kind white, ampicillin-sensitive colonies.

E) not grow.

14) An advantage of fabricated DNA over genomic or cDNA is the capacity to

A) insert desired restriction sites into the DNA sequence.

B) isolation unknown genes.

C) make DNA from cellular RNA and also the enzyme turning back transcriptase.

D) attain genes that lack introns.

E) acquire genes that absence exons.

15) An benefit of cDNA over genomic DNA is the it

A) lacks exons.

B) lacks introns.

C) consists of selectable markers.

D) can kind very huge DNA segments.

E) is really easy come isolate.

16) The border enzyme EcoRI establish the sequence G↓AATTC. Which of the following is TRUE that DNA after that is treated with EcoRI?

A) all of the DNA pieces will have actually single-stranded regions finishing in AA.

B) all of the DNA fragments will have single-stranded regions ending in G.

C) few of the DNA will have actually single-stranded regions ending in AA and others will finish in G.

D) all of the DNA will have blunt ends.

E) all of the DNA will be circular.

17) i m sorry enzyme would cut this strand that DNA?


A) Enzyme recognition



B) Enzyme acknowledgment



C) Enzyme recognition



D) Enzyme recognition



E) Enzyme acknowledgment



18) piece of DNA save in yeast cells are dubbed a

A) library.

B) clone.

C) vector.

D) southerly blot.


19) A populace of cells transferring a wanted plasmid is referred to as a

A) library.

B) clone.

C) vector.

D) southerly blot.


20) Self-replicating DNA supplied to transmit a gene indigenous one biology to an additional is a

A) library.

B) clone.

C) vector.

D) southerly blot.


21) The human Genome Project, which to be completed in 2003, was focused on

A) identifying all of the gene in the human being genome.

B) identify the nucleotide sequence of the entire human genome.

C) determining every one of the protein encoded by the person genome.

D) finding a cure for every human hereditary disorders.

E) cloning every one of the genes of the human being genome.

22) A colleague has used computer system modeling to architecture an improved enzyme. To produce this enzyme, the following step is to

A) look for a bacter that renders the enhanced enzyme. B) mutate bacteria until one renders the enhanced enzyme. C) recognize the nucleotide sequence for the enhanced enzyme. D) synthesize the gene for the enhanced enzyme. E) usage siRNA to produce the enzyme.

23) You have a tiny gene that you great replicated by PCR. ~ 3 replication cycles, how countless double-stranded DNA molecules perform you have?

A) 2

B) 4

C) 8

D) 16

E) thousands

24) i m sorry of the complying with places the steps in the PCR procedure in the correct order?

1) Incubate in ~ 94°C to denature DNA strands;

2) Incubate in ~ 72°C because that DNA synthesis;

3) Incubate at 60°C for primer hybridization.

A) 1, 2, 3

B) 3, 2, 1

C) 1, 3, 2

D) 2; 1; 3

E) 3; 1; 2

25) i beg your pardon of the adhering to are used to silence particular genes and also hold promise for dealing with cancer or famous diseases, such as hepatitis B?

A) RNA interference (RNAi)

B) security DNA (cDNA)

C) turning back transcriptase PCR (rtPCR)

D) tumor-inducing plasmids (Ti plasmids)

E) DNA fingerprinting

26) i beg your pardon of the adhering to techniques is NOT supplied to present recombinant DNA into plants?

A) gene guns

B) protoplast fusion

C) Ti plasmids and also Agrobacterium

D) microinjection

E) electroporation


27) In figure 9.5, the mite genes provided for choosing recombinant DNA are

A) HindIII, BamHI, and Eco

B) ampRand lacZ.

C) ori.

D) ampRand ori.

E) lacZ and ori.

28) In figure 9.5, the gene that allows the plasmid to be self-replicating is

A) Hind

B) ampR.

C) ori.

D) Eco

E) lacZ.

29) i m sorry of the complying with methods would be provided to present the plasmid displayed in number 9.5 right into E. Coli?

A) microinjection

B) transformation

C) gene guns

D) Ti plasmids and also Agrobacterium

30) A source of heat-stable DNA polymerase is

A) Agrobacterium tumefaciens.

B) Thermus aquaticus.

C) Saccharomyces cerevisiae.

D) Bacillus thuringiensis.


31) The Pap test because that cervical cancer involves microscopic examination of cervical cells for cancerous cells. A new, rapid diagnostic test to detect person papilloma virus (HPV) DNA before cancer develops is excellent without microscopic exam. The steps involved in this FastHPV check are listed below. What is the second step?

A) include an RNA probe because that HPV DNA.

B) Lyse person cells.

C) add enzyme-linked antibodies against DNA-RNA.

D) include enzyme substrate.

E) The order is unimportant.

32) Gene silencing involves every one of the complying with EXCEPT

A) little interfering RNAs.

B) production of dual stranded RNAs.

C) little interfering RNA binding come a gene promoter.

D) Dicer.

E) RNA-induced silencing complex.

33) You desire to recognize whether a person has actually a particular mutant gene. The procedure involves using a primer and also a heat-stable DNA polymerase. This process is

A) translation.

B) restriction mapping.

C) transformation.


E) site-directed mutagenesis.

34) i m sorry of the adhering to are supplied by the Centers for disease Control and also Prevention to track outbreaks the foodborne disease?

A) DNA fingerprints

B) border fragment size polymorphisms

C) reverse-transcriptase PCR (rtPCR)

D) DNA fingerprints and restriction fragment size polymorphisms

E) DNA fingerprints, border fragment size polymorphisms, and reverse-transcriptase PCR(rtPCR)

35) i think you have uncovered a cell that produces a lipase that functions in cold water because that a to wash additive. You can increase the efficiency of this enzyme by an altering one amino acid. This is done by

A) irradiating the cells.

B) site-directed mutagenesis.

C) enrichment.

D) selective breeding.

E) selection.

36) The usage of an antibiotic-resistance gene on a plasmid supplied in genetic design makes

A) replica plating possible.

B) direct an option possible.

C) the recombinant cabinet dangerous.

D) the recombinant cabinet unable come survive.

E) all of the answers space correct.

37) The following steps must be perform to do a bacterium create human protein X.


2-Restriction enzyme

3-Prokaryotic transcription

4-DNA ligase


6-Eukaryotic transcription

7-Reverse transcription

Which that the following places the procedures in the exactly order?

A) 5, 2, 3, 4, 7, 6, 1 B) 1, 2, 3, 5, 4, 7, 6 C) 6, 7, 2, 3, 4, 5, 1 D) 6, 7, 2, 4, 5, 3, 1 E) 6, 2, 1, 3, 4, 5, 7

38) The use of "suicide" gene in gene modified biology is design to

A) protect against the growth of the modified biology in the environment.

B) kill the modified organisms prior to they room released in the environment.

C) delete genes essential for amendment organism"s growth.

D) administer for resistance of the modified biology to pesticides.

E) provide a way to remove non-modified organisms.

39) A border fragment is

A) a gene.

B) a segment of DNA.

C) a segment of mRNA.

D) a segment that tRNA.

E) cDNA.

40) In the southerly blot technique, which of the adhering to is no required?

A) deliver of DNA come nitrocellulose

B) addition of a labeling probe to identify the gene of interest

C) border enzyme digestion of DNA

D) electrophoresis to different fragments

E) enhancement of heat-stable DNA polymerase to amplify DNA

41) i m sorry of the complying with processes is NOT affiliated in making cDNA?

A) turning back transcription

B) RNA handling to eliminate introns

C) transcription

D) translation

42) PCR deserve to be used to recognize an unknown bacterium because

A) the RNA inside wall is specific.

B) DNA polymerase will replicate DNA.

C) DNA can be electrophoresed.

D) all cells have actually DNA.

E) every cells have RNA.

43) The arbitrarily shotgun technique is provided in

A) amplification that unknown DNA.

B) transforming plant cells with recombinant DNA.

C) genome sequencing.

D) RFLP analysis.

E) forensic microbiology.

44) Restriction enzyme are

A) bacter enzymes that splice DNA.

B) bacterial enzyme that ruin phage DNA.

C) pet enzymes the splice RNA.

D) famous enzymes that ruin host DNA.

45) The examine of hereditary material taken straight from the atmosphere is

A) bioinformatics.

B) proteomics.

C) turning back genetics.

D) forensic microbiology.

E) metagenomics.

1) The ax biotechnology refers exclusively to the usage of genetically engineered organisms because that the production of desired products.

2) In recombinant DNA technology, a vector is a self-replicating segment of DNA, such as a plasmid or viral genome.

3) The practice of breeding plants and also animals for desirable traits, such as high crop yield, is called natural selection.

4) A spaceship vector is a plasmid that is supplied to move pieces the DNA among organisms, such together bacterial, fungal, and also plant cells.

5) virtually all cells, consisting of E. Coli and also yeast, naturally take up DNA from your surroundings there is no chemical treatment.

6) The disadvantage that genomic libraries end cDNA libraries is that genomic libraries contain gene introns.

7) one of the first commercial successes that recombinant DNA modern technology was the production of human insulin utilizing genetically engineered E. Coli.

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10) The Bt toxin acquired from Bacillus thuringiensis has actually been introduced right into some chop plants to do them resistant come insect destruction.